Variance-explained data for each of the 4 tissues were binned into 20 segments ranging from 0 to 1. Comparisons were then performed for NKT-NS versus NKT-LTS and ccRCC-NS versus ccRCC-LTS. Overlaid histograms were generated for these comparisons using R’s base statistics and graphics functions (hist, plot). For each comparison, statistical significance was obtained via the chisq.test function in R base statistics with a significance threshold at 0.05. The difference in variance explained for the 2 comparisons was computed for each metabolite pair, and the resulting values were sorted to find metabolite pairs that contributed the maximum degree of difference between the 2 tissues under consideration. The histogram distances were computed using the HistogramTools package in R and included Manhattan, Euclidean, Intersect distance, and Kullback-Leibler divergence.

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