Bacteria were harvested, washed, and resuspended in PBS just prior to use. Mice were anesthetized with isoflurane and 1 × 103 to 1 × 104 CFU of KP in a total volume of 100 μL was administered intratracheally under direct visualization using a sterile 200 μL pipet with filtered tip positioned just above the vocal cords. One hour after KP inoculation, 200 μL of sterile PBS, washed young RBCs (1 day old) or stored RBCs (11 to 13 days old) were transfused via retro-orbital vein.

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