RNA was extracted using RNAlater ICE (Ambion) and miRNA isolation kit (Ambion). The quality of RNA was checked using Bioanalyzer RNA 6000 Nano kit (Agilent). PolyA RNA was extracted using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and used as input for RNA-Seq. RNA-Seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB). After library quality control analysis using Bioanalyzer High Sensitivity DNA kit (Agilent) and library quantification using NEBNext Library Quant kit (NEB), the sequencing was performed under the setting of single read 1 × 51 bp to generate approximately 30 million reads per sample on HiSeq 1000 sequencer (Illumina).

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