KP strain 43816 serotype 2 from American Type Culture Collection (ATCC) was utilized for the bacterial pneumonia studies. entB ybtS mutant KP lacking production of siderophores Ent, gly-Ent, and Ybt and its parent WT strain have been previously described (32, 84). The method of bacterial growth and suspension for inoculation has also been previously reported (12).

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