Total RNA was extracted from sorted DCs and HSPCs using an RNeasy Micro Kit (QIAGEN) and reverse transcribed with a SuperScript VILO cDNA Synthesis Kit (Invitrogen). cDNA was quantified through quantitative real-time polymerase chain reaction (PCR) using SYBR Green PCR mix (Applied Biosystems) on an Applied Biosystems PCR system. Thermocycler conditions included an initial hold period at 95°C for 2 minutes; this was followed by a 3-step PCR program, as follows: 95°C for 30 seconds, 55°C for 30 seconds, and 72°C for 30 seconds for 40 cycles. Transcript abundance was calculated using the delta Ct method (normalization with 18S). The primer sequences are listed in Supplemental Table 5.

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