Tissue samples were fixed in cacodylate-buffered 2.5% (w/v) glutaraldehyde, postfixed in 2.0% osmium tetroxide, then embedded in epoxy resin. Ultrathin sections were poststained with uranyl acetate and lead citrate. Imaging was performed using a JEOL 1010 electron microscope fitted with a Hamamatsu digital camera and AMT Advantage imaging software. Micrographs were assessed by 2 investigators blinded to clinical diagnosis.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.