Acylcarnitines were measured as their butylated derivatives using tandem mass spectrometry. Stable isotope-labeled internal standards (Cambridge Isotope Laboratories) were added to samples. Ethanol was added and the samples were dried under a stream of nitrogen at 60°C. After adding butanolic hydrochloric acid (Regis Technologies), the samples were heated to 65°C for 15 minutes and dried under a stream of nitrogen. The dried samples were reconstituted with acetonitrile/water (80:20) and injected into a Xevo TQ-XS tandem mass spectrometer (Waters Corporation). Data were acquired to collect the parent compounds of mass m/z 85. Quantitation was against the nearest chain-length stable isotope labeled internal standard. The mass spectrometer was operated in positive ion mode with resolving power of 140,000 at m/z 200, mass scanning range being m/z 140–600, and 5 μL of sample was delivered to mass spectrometer through direct flow injection. Mobile phase was acetonitrile/water (80:20, v/v). Flow rate was 0.1 mL/minute. The source temperature was 150°C and desolvation temperature was 200°C, with gas flow of 550 L/hour. The cone and capillary voltage was 50 V and 3.2 kV, respectively. Data were acquired in multiple reaction monitoring (MRM). The mass spectrometer was operated in positive ion mode with resolving power of 140,000 at m/z 200, mass scanning range being m/z 140–600.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.