Animals were euthanized by CO2 asphyxiation followed by cervical dislocation. Colons were dissected, flushed with sterile PBS, and splayed open longitudinally and washed again with PBS. Tissue was incubated on ice in PBS containing 30 mM EDTA and 1.5 mM DTT for 20 minutes with occasional inverting. This was followed by shaking every 2 minutes in PBS containing 30 mM EDTA for 10 minutes to remove crypts from the submucosa. The mesenchyme was removed, and cells were pelleted by centrifugation at 400 g for 5 minutes at 4°C and washed in PBS.

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