Human colon cancer cell lines T84, Caco-2, and LS174T (ATCC) were maintained at 37°C in 5% CO2 in a humidified incubator. LS174T and Caco-2 cells were maintained in DMEM supplemented with penicillin-streptomycin and 10% FBS. T84 cells were maintained in DMEM/Ham’s F12 supplemented with penicillin-streptomycin and 10% FBS. Cultures were used for experiments when grown to confluent monolayers on 6-well plates unless otherwise noted.

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