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To specifically delete Vps33b in murine ECs, BM MSCs, Mks, OPCs, or spleen stromal cells, Vps33bfl/fl mice were crossed with Cdh5-Cre, Tie2-Cre, Cdh5-CreER, Lepr-Cre, Pf4-Cre, Osx-CreER, or Tcf21-CreER, respectively. CreVps33bfl/fl littermate mice served as controls. Tcf21-CreER transgenic mice and Cdh5-CreER knockin mice were generated by Beijing Biocytogen Co. Ltd. Six- to eight-week-old C57BL/6 CD45.2 mice, CD45.1 mice, and NOD/SCID mice were purchased from the Shanghai SLAC Laboratory Animal Co. Ltd. or Beijing Vital River Laboratories. Pirb–/– and Col2.3-GFP mice were kept in our institutes. All mice used in this study were 8–10 weeks old and female. To induce deletion by CreER, 4- to 6-week-old mice were given 1 mg tamoxifen (MilliporeSigma) in 100 μL corn oil (MilliporeSigma) daily by intraperitoneal injections for 5 consecutive days. All animals were maintained at the Animal Core Facility of Shanghai Jiao Tong University School of Medicine and the State Key Laboratory of Experimental Hematology.

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