All experiments were approved by the IACUC at the University of Pennsylvania Perelman School of Medicine. C57BL/6J, BALB/cByJ (SCAD deficient) and BALB/cJ (wild-type) were obtained from the Jackson Laboratory. Germ-free mice were obtained from the University of Pennsylvania Gnotobiotic Mouse Facility. All animals used in experiments were 7–10-week-old female mice. Animals were fed a standard chow diet and were kept on 12-hour light/12-hour dark cycles. For the C. rodentium model, conventionally housed, female, 8–10-week-old, C57BL/6J mice were inoculated by oral gavage with 6 × 108 CFU of C. rodentium (strain DBS100, gift from David Artis, Weill Cornell Medicine at Cornell University, New York, New York). Mice were euthanized by CO2 asphyxiation followed by cervical dislocation on day 11 after infection.

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