Total RNA from Lin cells was purified using the RNeasy Mini Kit (catalog 74104, QIAGEN) and used for RNA-Seq analysis at the Indiana University Center for Medical Genomics. The sequencing data were deposited in the NCBI’s Gene Expression Omnibus (GEO) public database (GEO GSE158351). For GSEA, differential gene expression was computed using DESeq2. Pathway enrichment analysis was computed using the Msigdb v6 canonical pathway, with P < 0.005 (or any other cutoff) as the significant cutoff. The log fold change of the expression level of each gene was used for GSEA. For RPKM plotting, the individual gene’s RPKM for the study groups was normalized to that of the WT group.

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