We performed a PCA comparing gene expression in the 2 tissues and analyzed expression profiles of genes in 16 disease classes reflecting the varied phenotypes of probands enrolled in the BCM UDN site (Supplemental Table 1), and considered well-expressed genes as those having a minimum of 10 TPM (38). We determined the number and percentage of genes well expressed for each respective disease class in aggregate across all samples and compared the statistical difference between tissues using the 2-sample t test in R (version 3.6.0) (50). The 95% CIs were calculated for the reported diagnostic rates with the binomCI function (51) in R using the Wald method.

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