Primary Schwann cell cultures were prepared as previously described (55) with minor modifications. Briefly, Schwann cells were obtained from the sciatic nerves of postnatal 2-day-old Sprague-Dawley rat pups. Contaminating fibroblasts were removed from the culture by treating the cells with 10 μM cytosine arabinoside for 48 hours and complement-mediated cytolysis using anti-Thy1.1 (Serotec) and rabbit complement (Cappel). Schwann cells were propagated on poly-L-lysine–coated plates in DMEM supplemented with 10% FBS, 2 μM forskolin, and 20 ng/mL rh-HRG-β1 (MilliporeSigma). Schwann cells were stimulated with DMEM containing 10% FBS and 500 ng/mL BMP3B for 4 days and lysed in lysis buffer (Qiagen) for gene expression analysis.

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