For VSV infection, 1.25 × 105 SV40-fibroblasts per well were added to 24-well plates and infected with VSV (Indiana strain), at an MOI of 3, in DMEM supplemented with 2% FCS. After incubation for 2 hours, the cells were washed and incubated in 1 mL of medium. Cells and supernatants were obtained at various time points and frozen. VSV titers were determined by calculation of the 50% end point (TCID50), as described by Reed and Muench (97), after the inoculation of Vero cell cultures in 96-well plates. For HSV-1 (KOS strain, ATCC) and EV71 (ATCC) infection, 1.25 × 105 SV40-fibroblasts per well were added to 24-well plates and infected with HSV-1 or EV71, at a MOI of 0.01 or 10, respectively, in DMEM supplemented with 2% FCS. After 2 hours, the cells were washed and incubated in 500 μL of medium. Cells and supernatants were collected at various time points and frozen. HSV-1 titers were determined by calculation of the TCID50, as described by Reed and Muench, after the inoculation of Vero cell cultures in 96-well plates.

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