RNA was isolated from PBMCs, EBV-B cells, fibroblasts, or HEK293T cells with and without plasmid transfection, with a kit, according to the manufacturer’s protocol (Zymo Research), and treated with DNase (Zymo Research). Reverse transcription was performed with random hexamers and the SuperScript III reverse strand synthesis kit, according to the manufacturer’s instructions (Thermo Fisher Scientific). Real-time qPCR was performed with Applied Biosystems TaqMan assays for IFNAR1, and the β-glucuronidase (GUS) housekeeping gene for normalization. Results are expressed according to the ΔΔCt method, as described by the kit manufacturer.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.