RNA was isolated from PBMCs, EBV-B cells, fibroblasts, or HEK293T cells with and without plasmid transfection, with a kit, according to the manufacturer’s protocol (Zymo Research), and treated with DNase (Zymo Research). Reverse transcription was performed with random hexamers and the SuperScript III reverse strand synthesis kit, according to the manufacturer’s instructions (Thermo Fisher Scientific). Real-time qPCR was performed with Applied Biosystems TaqMan assays for IFNAR1, and the β-glucuronidase (GUS) housekeeping gene for normalization. Results are expressed according to the ΔΔCt method, as described by the kit manufacturer.

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