Z-stack images of 40 μm thick longitudinal TA sections were captured using the TCS SP8 confocal laser scanning microscope system (Leica), and reconstructed images were displayed by maximum intensity projection using LAS X software (Leica). For denervation assessment, we analyzed 50–100 NMJs per TA muscle. The numbers of completely denervated, partially denervated, and innervated NMJs were counted.

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