The pCAGGS-IFNAR1 plasmid was used to transfect HEK293T cells, HeLa cells, or SV40-fibroblasts, by incubation for 24 hours or 48 hours, in the presence of X-tremeGene 9 transfection reagent (Sigma-Aldrich). We used 1 μg of plasmid to transfect 0.5 × 106 cells. For stable transduction of the patient fibroblasts with WT IFNAR1, HIV-based vectors were generated as previously described (42) and used to transduce SV40-fibroblasts in a serial dilution series. Cells were cultured and subjected to blasticidin selection (5 μg/mL). IFNAR1 expression was corroborated by Western blotting.

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