C57BL/6 mice and GFP-Tg mice were purchased from Japan SLC. Pdgfra-CreER mice (19) (stock 018280), R26-EYFP mice (46) (stock 018280), and R26-DTA mice (47) (stock 010527) were purchased from The Jackson Laboratory. Bmp3b/Gdf10-KO mice (26) were provided by Se-Jin Lee (Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA). CAG-CAT-mBmp3b–Tg mice have previously been described (27). Genetically engineered mice were backcrossed to C57BL/6 mice at least 6 times. Tmx (4 mg, MilliporeSigma) was injected intraperitoneally for 5 consecutive days at 10–11 weeks of age to induce recombination. Thirteen-week-old male Bmp3b/Gdf10-KO and WT littermate mice were used, while female mice were used in other experiments. We used 8- to 12-week-old C57BL/6N females as young mice and 24- to 28-month-old C57BL/6N females as aged mice.

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