The complex I pulldown assay was performed using Dynabeads protein A IP kit from Invitrogen. Dynabeads were coated with 1 μg anti–complex I immunocapture antibody from Abcam (ab109798, 18G12BC2) or 1 μg of the negative control IgG (Sigma-Aldrich). Total proteins from A549 cells were extracted with a RIPA buffer. IP experiments were realized with positive control such as purified IgG and negative control using beads without antibody coating. Western blot was performed as previously described (18). Proteins were detected using primary antibodies against HADHA (Abcam, 203114).

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