Statistical analyses were performed using GraphPad Prism version 7. P values less than 0.05 were considered significant: *P = 0.01 to 0.05, **P = 0.001 to 0.01, ***P = 0.0001 to 0.001, and ****P < 0.0001. All data are shown as mean ± SEM. All statistical tests corrected for multiple comparisons when there were more than 2 groups in an experiment.

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