Total RNA was treated with DNase and subjected to cDNA synthesis using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems). qPCR was performed in triplicate. RNA levels were expressed as fold changes after normalization to Gapdh RNA. Sequences of primers used for qPCR are listed in Supplemental Table 8.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.