Methods from Anderson et al. were followed for these experiments (29). Mice were injected with anti-CD41 BV421(MWReg30, BioLegend) i.v. and then sacrificed after 3 minutes. The organs and blood were then stained ex vivo with anti-CD41 BV786 (MWReg30, BD Biosciences), allowing the discrimination of tissue and vascular Mks.

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