Total RNAs (including mRNA and miRNA) were extracted from cultured cells or mouse ears using TRIzol Reagent (Invitrogen). For measurement of human galectin-7, IL-6, IL-8, IL-17A, and IFN-γ mRNA levels, mRNAs were reverse-transcribed into cDNA by means of the iScript cDNA Synthesis Kit (Bio-Rad), and real-time PCR was carried out, using specific Universal Probe Library probes (Roche) accompanied by primers targeting these gene products, according to the manufacturer’s instructions.

For miR-146a quantification, total RNA samples were converted to cDNA, and real-time PCR was conducted with specific primers targeting miR-146a using the Mir-X miRNA First-Strand Synthesis and SYBR qRT-PCR Kit (Clontech). Human GAPDH and U6 snRNA were selected as internal controls for normalization of the levels of mRNAs and miRNAs, respectively. Relative levels of mRNAs and miRNAs were calculated, and fold changes were obtained using the ΔΔCt method and compared with vector-only control cells.

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