Total RNA was extracted from tumor cells using the Direct-zol RNA Kit (Zymo) and reverse transcription was performed using the iScript Advanced cDNA Synthesis Kit (Bio-Rad). Quantitative real-time PCR was utilized to detect mRNA expression using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) and TaqMan probes (Thermo Fisher Scientific, Mm00451763_m1 for Noxa, Mm00432051_m1 for Bax, Mm00519268_m1 for Puma, Mm01253561_m1 for NQO1, Mm99999915_g1 for Gapdh). Gapdh expression was used as an internal control for RNA concentration across samples. Every sample was run in triplicate, and the results were averaged for each assay.

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