Age matched, 6–7-week-old male mice were anesthetized with i.p. injection of a mixture of ketamine (100 mg kg-1 body weight) and xylazine (10 mg kg-1 body weight). We tested mice of both genders and did not find any gender differences in any of the readouts used in the current study. Both corneas were scarified in a 10 × 10 crosshatch pattern with a 25 gauge needle and mice inoculated with HSV-1 (strain McKrae, the dosage used is indicated in the figure legends) in 5 μl of infection medium (DMEM containing 200 IU mL-1 penicillin and 200 mg mL-1 streptomycin), or mock-infected with 5 μl of infection medium. Mice were scored for disease and weighed at the indicated times after infection. The scoring was performed as a blinded study, with head swelling scored as 0: none, 1: minor swelling, 2: moderate swelling, 3: severe swelling.

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