C57BL/6 (WT), Stinggt/gt, and cGas–/– mice were bred at Taconic M&B and at University of Aarhus (Denmark); Irf3–/–, Irf3s1/s1, and Ifnar–/– mice were only bred at University of Aarhus. Ifnar–/–, Stinggt/gt, and cGas–/– mice were purchased from the Jackson Laboratory. Irf3–/– and Irf3s1/s1 mice were both gifts from Ganes C. Sen, Department of Immunology, Cleveland Clinic, Cleveland, Ohio, USA. Isoflurane (Abbott) or a mixture of ketamine (MSD Animal Health) and xylazine (Rompun Vet) were used to anesthetize mice. All described animal experiments have been reviewed and approved by the Danish government authorities and hence comply with Danish laws. All efforts were made to minimize suffering and mice were monitored daily during infection. The mice were not randomized, but after HSV-1 infection, the information about mouse strain and treatment was blinded to the investigators. No animals were excluded from the analysis. Chow and water were provided ad libitum.

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