Human artery–NSG mouse chimeras were generated as previously described (3). Briefly, NSG mice (from The Jackson Laboratory) were used at the age of 8 weeks. Human temporal and axillary arteries (diameters of 4–5 mm and 6–9 mm, respectively) were cut into fragments and transplanted subcutaneously into the back. Chimeras were i.p. injected with LPS (10 μg/mouse) on day 7 and reconstituted with PBMCs from GCA patients (10 million/mouse) on day 8. Mice transplanted with the arteries from the same donor were randomly assigned into 2 groups receiving control or genetically manipulated CD8+ T cells. CD8+ Treg cells (5 million/mouse) were coadministered with patient-derived PBMCs on day 8. Arterial grafts were harvested on day 15 and processed for RNA extraction or embedded for IHC analyses. For all animal experiments, the investigators performing the tissue analysis were blinded to the treatment assignment of the mice. All protocols were approved by the Stanford University IACUC.

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