Cells were fixed with Fix I buffer (BD Phosflow) and permeabilized with Perm buffer III (BD Phosflow), followed by staining with primary antibodies (4°C, overnight) plus secondary antibodies (room temperature, 2 hours). Cell nuclei were stained with DAPI in mounting medium (Sigma-Aldrich). Cells were visualized using the LSM710 confocal microscope (Carl Zeiss) with a Plan-Neofluar ×63/1.3 NA oil objective lens. All primary and secondary antibodies are listed in Table 2.

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