Total mRNA was extracted with a Direct-zol RNA MiniPrep Kit (Genesee Scientific). cDNA was synthesized using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Real-time PCR was performed with SYBR Green qPCR Master Mix (Bimake) and β-actin was used as an internal control. All primers are listed in Table 3.

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