H1299 cells in a 6-well plate were transfected with siRNAs against G55 or control siRNA. After 48 hours, cells were incubated in free medium for 16 hours. CM samples were collected and subjected to ELISA assay to quantify IL-6 concentrations using a Human IL-6 ELISA Kit (ab46027, Abcam) according to the manufacturer’s instructions.

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