A549 cell lysates were formaldehyde fixed and digested using SimpleChIP Enzymatic Chromatin IP Kit (9002, Cell Signaling Technology) following the manufacturer’s instructions. Resulting chromatin was immunoprecipitated with anti–RNA polymerase II or anti–rabbit IgG antibodies (Santa Cruz Biotechnology). DNA was eluted and purified with the MinElute Reaction Cleanup Kit (Qiagen) and subjected to quantitative PCR. PCR primers are listed in Supplemental Table 4.

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