PBMCs from GCA patients and healthy controls were isolated by gradient centrifugation with Lymphoprep Medium (Cosmo Bio USA) and cultured in RPMI 1640 (Thermo Fisher Scientific) medium with 10% FBS (Gemini) plus penicillin/streptomycin (Thermo Fisher Scientific). Naive CD4+ T cells were purified from PBMCs using EasySep human naive CD4+ T cell enrichment kits (STEMCELL Technologies, Inc.). Purity of cell populations was consistently greater than 90%.

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