PBMCs from GCA patients and healthy controls were isolated by gradient centrifugation with Lymphoprep Medium (Cosmo Bio USA) and cultured in RPMI 1640 (Thermo Fisher Scientific) medium with 10% FBS (Gemini) plus penicillin/streptomycin (Thermo Fisher Scientific). Naive CD4+ T cells were purified from PBMCs using EasySep human naive CD4+ T cell enrichment kits (STEMCELL Technologies, Inc.). Purity of cell populations was consistently greater than 90%.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.