As previously described (39), HUVECs (1 × 105/well) were seeded into the lower chambers of Transwell plates containing Matrigel-coated filters, and tumor cells (2 × 104/well) were seeded in the upper chambers. After 8 hours, HUVECs were imaged and tube-like structures were quantified.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.