We purchased SYBR Green, FBS, HEPES-buffered media, DMEM, RPMI 1640, Alexa Fluor–tagged secondary antibodies, and DAPI from Life Technologies; puromycin from InvivoGene; paraformaldehyde from Electron Microscopy Sciences; Transwell and Matrigel-coated Boyden chambers from BD Biosciences; G418 from Corning; GRASPIN (PubChem CID: 3113208) from Vitas-M Laboratory (STK700118); and wortmannin (W1628), 3-methyladenine (SAE0107), nutlin-3 (444151), and brefeldin A (B7651) from MilliporeSigma. We purchased the following from MilliporeSigma: shRNAs against murine G55 (TRCN0000278363,TRCN0000077518, and TRCN0000077520) and murine G45 (TRCN0000096503), shRNAs against human G55 (TRCN0000278363 and TRCN0000130898), human IGFBP2 (TRCN0000318660, TRCN0000318716, TRCN0000318659, and TRCN0000006576), human SPP1 (TRCN0000342616, TRCN0000342561, TRCN0000004878 and TRCN0000004875), human G45 (TRCN0000013243); siRNAs against human G55 (SASI_Hs01_00012371 and SASI_Hs02_00348040), human IGFBP2 (SASI_Hs02_00302878 and SASI_Hs01_00039595), human SPP1 (SASI_Hs01_00174866 and SASI_Hs01_00174867), human G45 (SASI_Hs01_00073080 and SASI_Hs01_00073083), human MYOIIA (SASI_Hs01_00197338 and SASI_Hs01_00197339), human MYO18A (SASI_Hs01_00224320 and SASI_Hs01_00224321), GOLPH3 (SASI_Hs02_00355527 and SASI_Hs01_00133692); esiRNAs against human RAB6A (EHU113451), human RAB8A (EHU054251), human RAB10 (EHU131761), human RAB13 (EHU107931), and EGFP (EHUEGFP). We purchased primary antibodies against G55 (NB100-74431) from Novus Biologicals; against GM130 (560066) from BD Transduction Laboratories; against G45 (OAAN01805) from Aviva Systems Biology; against GOLPH3 (ab98023) from Abcam; against IGFBP2 (11065-3-AP), SPP1 (22952-1-AP), MYO18A (14611-1-AP), MAOA (10539-1-AP), and RAB6A (10187-2-AP) from Proteintech; against α-tubulin (T9026), EGFP (G6539) from MilliporeSigma; against PARP-1 (9542), hemagglutinin (3724 and 2367), β-actin (4970), p53 (9282), GM130 (12480), EEA1 (3288), ubiquitin (43124), and MYOIIA (3403) from Cell Signaling Technology; against ACBD3 (sc-101277), GRASP65 (sc-374423), and p53 (sc-126) from Santa Cruz Biotechnology; against calnexin (ab22595) and RAB11 (ab95375) from Abcam; and against RNA polymerase II (05-623, clone CTD4H8) from MilliporeSigma. We purchased neutralizing antibodies against IGFBP2 (AF674) and SPP1 (AF1433) from R&D Systems. We purchased recombinant IGFBP2 (ab63223) and SPP1 (ab92964) proteins from Abcam. We purchased a human IL-6 ELISA kit (ab178013) from Abcam. rG55-pEGFP-N3 was a gift from Yanzhuang Wang at the University of Michigan Medical School. Myosin-IIA-GFP was a gift from Matthew Krummel (Addgene plasmid 38297). EGFP-RAB6A was a gift from Marci Scidmore (Addgene plasmid 49469).

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