WT and IFT88-KOSF-1 male littermates were examined for whole-body fat and lean masses, BMD, BMC, BV, and BA using DEXA (InAlyzer, MEDIKORS) under light isoflurane anesthesia for about 1 minute. The detection sensitivity of the DEXA instrument was 0.001 g/cm2. A standard calibration block was used to calibrate the DEXA device before measurements in accordance with the operator’s manual. Software integrated to the scan was used for data analysis.

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