4-Hydroxytamoxifen (MilliporeSigma) was dissolved in 100% ETOH at 4 mg/mL. P0 pups were injected subcutaneously with 40 μg/μL 4-hydroxytamoxifen. For adult mice, tamoxifen (MilliporeSigma) was dissolved in a sunflower oil/ethanol mixture (9:1) at 10 mg/mL. The mice were gavaged with 2 mg/200 μL tamoxifen for 5 consecutive days. Mice were genotyped before and after injection to confirm the deletion of genes flanked by loxP sites using the Nf1 primers listed in Supplemental Table 6.

Supplemental Table 7 lists the different genotypes of the mice and their phenotypes.

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