To determine the genotypes of the genetically modified mice, genomic DNA was extracted from tails (pups) using 1 M Tris-HCl (pH 6.8) and incubated for 1 hour followed by 50 mM NaOH neutralization. The DNA sequences including the target site were amplified with 2XTaq RED Master Mix (Apex). The primer sequences are listed in Supplemental Table 6.

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