To silence TP53 expression in NF1–/– hiPSCs, annealed sgRNAs targeting TP53 were ligated to a digested CRISPRv2 vector. After transformation of the ligation product into Stbl3-competent cells and screening single clones on ampicillin agar plates, the resulting clone was expanded in Luria-Bertani (LB) broth, and plasmids were extracted using NucleoBond Xtra Midi (Macherey-Nagel). The sgRNA primers used in this study are listed in Supplemental Table 5.

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