Twelve million BM cells were harvested from young and old C57BL/6 mice (CD45.1CD45.2+). Cells were injected i.v. into lethally irradiated (950 cGy, 24 hours prior) B6 Boy/J F1 (CD45.1+CD45.2+) recipient mice while under either hypoxic or ambient air conditions as previously described in the transplantation section. Eighteen hours following injection, BM from the recipient mice was collected and analyzed by flow cytometry for the presence of CD45.1CD45.2+ LSK CD150+ cells (see Figure 3G for the experimental design).

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