Protein extracts were separated by 10% SDS-PAGE and transferred onto nitrocellulose membranes (Invitrogen). Blocking and antibody incubations were performed in 5% BSA. Membranes were incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (Jackson ImmunoResearch) for 1 hour at room temperature. Antibody detection reactions were developed by enhanced chemiluminescence (MilliporeSigma).

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