Heterozygous p.H222P/+ and homozygous p.H222P/H222P E13.5 embryos were collected from pregnant heterozygous p.H222P/+ females crossed with homozygous p.H222P/H222P males. Embryos were genotyped using the yolk sac. The hearts were dissected, and RNA was extracted using a Zymo Research kit. RNA was then used to generate a sequencing library or in real-time qPCR. RNA was sequenced using a NextSeq (Illumina). Sequence alignment using the mouse GRCm38/mm10 genome reached 94.91% and included 97.95% of intragenic regions. The data were analyzed using the DESeq2 application. Data are available in NCBI’s Gene Expression Omnibus database (GEO GSE156975).

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