Heterozygous p.H222P/+ and homozygous p.H222P/H222P E13.5 embryos were collected from pregnant heterozygous p.H222P/+ females crossed with homozygous p.H222P/H222P males. Embryos were genotyped using the yolk sac. The hearts were dissected, and RNA was extracted using a Zymo Research kit. RNA was then used to generate a sequencing library or in real-time qPCR. RNA was sequenced using a NextSeq (Illumina). Sequence alignment using the mouse GRCm38/mm10 genome reached 94.91% and included 97.95% of intragenic regions. The data were analyzed using the DESeq2 application. Data are available in NCBI’s Gene Expression Omnibus database (GEO GSE156975).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.