Eight- to 12-week-old female C57BL/6 (B6, H-2b) mice were purchased from The Jackson Laboratory or Harlan Laboratories. Act-2W-OVA transgenic mice on a C57BL /6 background (2W-OVA.C57BL/6) were a gift from James Moon (Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA). Secretory IgM (Ighm) and AID (Aicda) double-knockout mice (H-2b, sIgKO) mice were originally a gift from Frances E. Lund (38). B6.129S2-Igh-6tm1Cgn/J (μKO) mice were purchased from The Jackson Laboratory. Donor 2W-OVA.BALB/c mice were backcrossed from 2W-OVA.C57BL/6 mice for more than 10 generations. For mating, male 2W-OVA.BALB/c mice were introduced to virgin C57BL/6 females, and successfully mated mice were confirmed by a visualized copulation plug representing E0.5. Approximately 50% of F1 from this mating were confirmed to be 2W-OVA positive. In other experiments, male 2W-OVA.BALB/c were mated with female sIgKO or μKO mice.

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