In each daily session mice had two 10-minute periods of access to the sipper tube separated by an interval of approximately 10 min in which mice were returned to the home cage. On half of the sessions, mice had 10 min of access to 1% sucrose (with no flavour) and then 10 min of access to the CS+ flavour mixed with 4% sucrose. On the other half of sessions, mice had 10 min of access to 16% sucrose (with no flavour) followed by 10 min of access to the CS–flavour mixed with 4% sucrose. Mice received eight days of training, four with each flavour. Flavours were presented in a double alternating order (i.e., XYYX) across days. On the ninth day mice received a test session. Similar to training sessions, mice received two 10-min periods of access to the sipper tube. The CS– and CS+ flavours mixed with 4% sucrose were presented, one in each 10-minute period of access. The allocation of flavours to conditions (CS+ or CS–), the order of flavours and conditions during training and within the test session was counterbalanced within genotype and sex as far as possible given the number of mice within each subgroup.

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