Flow cytometry analysis was performed on a FACSCanto II and FlowJo software (both from BD Biosciences). Dead cells were excluded using the fixable viability-dye-eFluor780 (Thermo Fisher Scientific), and nonspecific FcR-mediated staining was blocked with blocking reagent (Miltenyi). Stainings were performed for 15 minutes at 4 °C with fluorophore-labeled antibodies as listed in Supplementary Table S1 (Supplementary Material). Fluorescence minus one or isotype-matched antibodies were used as controls. For intracellular staining, Cytofix/Cytoperm kit (BD Biosciences) was used according to the manufacturer’s instructions.

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