Previous studies have shown that chronically damaged axons show accumulation of nonphosphorylated neurofilaments H which is revealed with SMI32 immunoreactivity (Lindner et al., 2009). As described previously (Craggs et al., 2014), SMI32-stained sections from CADASIL and young and older controls were viewed under an Olympus BX51 upright microscope coupled with an mbf Bioscience CX9000 camera and analyzed with a Stereo Investigator (MBF Bioscience, VT, USA) using the Space Balls probe. SMI32 axons were examined in the whole temporal stem WM region as the ROI (Fig. 1).

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