Breast cancer cells transfected with scramble shRNA or USP37#1 shRNA were seeded in a 96-well plate at a density of 5×103 cells/well. With different concentrations of adriamycin pre-treatment for 24/48 hrs, the cells were cultured in a mixed liquid, containing 10 μl of CCK-8 and 90 μl of fresh serum-free medium. The absorbance levels of samples were measured by a Microplate Reader at 490 nm. Cell viability was calculated according to the manufacturer's instructions 9.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.