Murine bone marrow-derived dendritic cells (BMDCs) were prepared as previously described 16. Briefly, BMDCs were cultured with RPMI media containing GM-CSF (20 ng/mL), 1% penicillin/streptomycin, 1% L-glutamine, 10% FBS, and 2-mercaptoethanol (0.1 μg/mL) in a 5% CO2 incubator at 37°C, and fresh media was added on days 3 and 6. After 9 days, non-adherent cells were collected by vigorous aspiration. For cytokine measurement, the cells were seeded in 48-well plates at a concentration of 2×105 cells/well and incubated in a 5% CO2 incubator at 37°C for 2 h. Subsequently, the cells were infected with P. nigrescens at the indicated multiplicity of infection (MOI) (uninfected cells were used as controls). Culture supernatant was collected 24 h after infection for cytokine measurement.

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