Studies compared PIGR between cancer and normal samples in liver cancer were selected with analysis type by “liver cancer vs. normal”, gene name by “PIGR”, threshold by p-value ≤ 1E-4, fold change ≥ 2 and top 10% gene rank in Oncomine database (https://www.oncomine.org/). Profiles including Guichard Liver, Guichard Liver 2 and TCGA liver were included in this analysis. In order to validate the PIGR expression levels in serum and tissues HCC and healthy candidates, Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) database was searched using heading terms including “liver cancer” and “hepatocellular carcinoma”. All the series with expression profiling by array were included and homo sapiens were selected. Platforms with terms including “ID”, “Gene Symbol” and “ENTREZ_GENE_ID” were also restricted. GEO profiles including GSE49515 14, GSE55092 15 and GSE60502 16 were included in this analysis. Additionally, PIGR protein detected by immunohistochemistry was obtained from the Human Protein Atlas (HPA) databases (http://www.proteinatlas.org) 17-19.

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