The transfected cells (1×106 cells per well) were seeded into 6-well plates and incubated for 24 h at 37 °C under indicated treatment. The culture medium was collected and subjected to a glucose assay kit (ab136955, Abcam, Cambridge, MA, USA), lactate assay kit (ab65331, Abcam), and pyruvate assay kit (ab65342, Abcam) for measuring glucose consumption, lactate production, and pyruvate production according to the manufacturer's instructions.

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